FastQCFastQC Report
Fri 10 Feb 2017
SRR3557945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520667
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556181.579757472092646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268110.761531834734725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217520.6178374722744298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110160.31289525535928275No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93520.2656314840341333No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT77610.22044118344620492No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77610.22044118344620492No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC75420.21422077123454164No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC67260.1910433449116318No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT64270.182550636001644No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA53490.15193143799172146No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48190.1368774723653217No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA47490.13488921275428775No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT46400.1317932085028206No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45160.12827114862041766No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41910.11903994328347442No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT41820.1187843099049129No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA41530.11796060235177026No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40290.11443854246936731No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37510.1065423114426897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11250.059.746591
TAACGCA8800.043.8964234
AGATTAC15200.043.4025152
ATAGTAC15900.042.2357064
TAGTACT16000.041.9717375
TACAACG6800.041.9534652
GTATAAC9450.040.91371
GCATAGT17000.039.5061762
ATAACGC10300.037.503743
AACGCAG593350.036.4376376
GTATCAA575100.036.283021
ACAACGC8700.033.4715083
GTACTGG30550.033.0997661
GACCGTT7900.033.0996977
CATGGGC137350.032.6675644
ACGCAGA663200.032.6268127
CGCAGAG666550.032.569828
TACTGGT30100.032.3826832
CATGGGG127000.032.28824
TATCAAC643600.032.2473222
Produced by FastQC (version 0.11.5)