FastQCFastQC Report
Fri 10 Feb 2017
SRR3557936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730277
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288901.058134394422251No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132340.48471272328778364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122130.4473172502277242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59590.21825624286473497No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51180.18745350746462722No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA45820.16782179976610434No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34300.1256282787424133No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA31530.11548278801015428No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.10566693416089283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7400.057.886841
TAACGCA6750.049.3071374
TATCACG3850.043.2277452
TACAACG5550.040.6963962
ATAACGC8450.038.684013
ACGCATA3650.037.4898831
GTATAAC9250.037.3048551
ACAACGC6200.035.4679033
TAGTACT12300.034.3066865
CATGGGG86800.034.235434
AACGCAG322250.034.101196
GCATAGT13050.032.793462
GTACATG335450.032.651651
ACATGGG330800.032.429273
TACATGG338350.032.4287952
ATAGTAC13050.032.3350334
GTATCAA329700.031.9579331
ACGCAGA360050.030.5210637
CGCAGAG361600.030.4395398
CATGGGC83250.030.1268524