FastQCFastQC Report
Fri 10 Feb 2017
SRR3557923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6452456
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT702931.089399137320735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350090.5425685971357264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263240.40796868665202835No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA238570.3697351830062847No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT198700.30794475777905345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153500.23789391202357676No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG126020.19530547748020288No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC111840.17332934932063077No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA108310.16785856424282475No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG98030.1519266462258712No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA86590.13419696314085675No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT85560.13260067174421647No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG84880.1315468094629394No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC71230.1103920739637744No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66780.10349547521129938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG15900.065.877181
TACAACG13650.047.9124642
TATCACG10650.043.5445142
ACAACGC15650.042.9271773
TAACGCA17100.041.3709564
TAGTACT28500.037.964525
ATAGTAC28000.037.792594
AGATTAC31850.036.4010282
GCATAGT31050.034.083712
TCACGCA14350.033.5565874
ATCACGC14050.033.428663
GTACAAC23050.032.7908171
CATGGGT98350.032.641054
GATTACT36050.032.1586343
GTATAAC24150.031.2972411
CATGGGG180500.030.6302414
AAACGCA18750.029.4871715
AACGCAG1027700.026.6966516
ATGGGTG61950.026.390215
GTATCAC24100.026.17631
Produced by FastQC (version 0.11.5)