FastQCFastQC Report
Fri 10 Feb 2017
SRR3557915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393999
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498212.0810785635248803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212830.8890145735232136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204720.8551382018121143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87030.36353398643859086No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50950.21282381488045735No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA42370.17698420091236464No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT39880.16658319406148459No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.16060992506680244No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC36620.15296581159808337No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC36410.152088618249214No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.12773606004012533No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA29470.12309946662467278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC28670.11975777767659886No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT27070.11307439978045103No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26910.11240606199083626No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA24410.10196328402810528No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA24280.10142025957404326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA346300.054.2395671
GACCGTT5550.048.204597
AACGCAG416600.045.46696
TATCAAC422250.044.382592
GGACCGT7900.043.648556
ATCAACG432150.043.2961463
TCAACGC442550.042.505294
CAACGCA450250.041.8179935
ACGCAGA477250.039.713787
CGCAGAG479300.039.531528
GCAGAGT497500.038.0733879
GGTATCA187400.037.189391
TACTGGT18350.036.6132662
GTACTGG20450.035.5057141
ACTGGTT19700.032.8963173
ATGGGAC36750.032.8403325
CATGGGT54950.032.5662424
GTACATG390300.031.9613021
TACATGG391750.031.841432
ACATGGG389950.031.6523133