FastQCFastQC Report
Fri 10 Feb 2017
SRR3557876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2865219
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239550.836061746065484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112490.39260524239159383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99630.3477221112941105No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77540.27062503773708046No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55960.1953079328316614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42900.14972677481197774No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42040.14672525904651618No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC36570.12763422272433625No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35440.12369037061390421No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33500.11691950946856068No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33380.11650069331524049No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30490.10641420428944524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA5950.049.9733474
CGCATAG5550.048.2630041
TACAACG6600.036.042642
GTATAAC10350.031.0561961
ACAACGC7550.030.7187163
AAACGCA8550.029.9090715
CATGGGG91450.029.4577714
AACGCAG372550.027.9832366
GTATCAA360750.027.8027921
AGATTAC13350.027.1737212
TAGTACT10200.026.8199675
ACATGGG335150.026.5269533
ATAACGC12250.026.2145883
ACGCAGA402600.025.9388837
TATCACG5050.025.9078792
CGCAGAG406300.025.7319458
GTACAAC10700.025.5899181
GACCGTT5600.025.4872957
ATAGTAC10750.025.4468944
CATGGGT34150.025.4242134
Produced by FastQC (version 0.11.5)