FastQCFastQC Report
Fri 10 Feb 2017
SRR3557872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12780927
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1746501.3664893008151913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT825820.6461346661318073No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA677870.5303762395325472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT647240.5064108417175062No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT602860.4716872258170319No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336670.263415947841655No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG302290.23651649054876847No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT277170.2168622041265082No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG267480.2092805944357557No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC224240.1754489326165465No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC198650.15542691073972958No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG185900.14545110851505527No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC184380.14426183640670195No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC177010.13849543151290983No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA170990.13378528803114204No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165120.12919250692848805No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT157880.12352781609659456No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC148140.11590708561280413No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC139280.10897488108648146No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA137640.10769171907483706No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA130250.10190966586382975No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA128740.10072821791408401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG26800.050.422091
TACAACG23000.035.6824232
GACCGTT22900.035.059567
TAACGCA29850.031.4782624
ACAACGC27350.030.8765663
GGACCGT40000.030.4790926
TCACGCA23250.030.438414
ATAGTAC46300.030.184564
TAGTACT47300.029.2956375
CATGGGG452150.028.8439064
CATGGGT195750.028.5881374
CATGGGC393250.027.9164984
GCATAGT50650.027.4751152
AGATTAC56400.027.0992372
ATAACGC43550.027.0380293
GTATAAC40400.026.9649411
GTATCAA2510850.026.826471
AACGCAG2594550.026.8091776
GTACTGG96150.026.746331
TATCACG24300.026.186692
Produced by FastQC (version 0.11.5)