FastQCFastQC Report
Fri 10 Feb 2017
SRR3557847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7085919
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT893161.2604716480671032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405990.5729532047995468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340450.4804599092933464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152780.21561070624713605No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA117030.1651585348350722No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC106360.15010050213670237No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT104080.1468828531627302No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC99570.14051811769228523No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT88200.12447221030892394No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79060.11157338942203544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA848500.040.4677281
AACGCAG907450.038.3686146
CGCATAG10350.037.3859561
TACAACG12750.034.9859852
TATCAAC996100.034.3804632
ACGCAGA1018550.034.201017
CGCAGAG1024950.034.0570878
ATCAACG1007950.033.8983653
TCAACGC1026400.033.5787474
CAACGCA1037600.033.2578245
GCAGAGT1073200.032.5314569
ACGGTAC18250.031.6108783
CATGGGT129100.030.036484
CATGGGG237900.028.8245534
GACCGTT13650.028.7579127
ACATGGG837550.028.2476123
GTACATG884900.027.7703271
ACAACGC15450.027.7160723
TACATGG888950.027.6188372
GGACCGT19100.026.7800376