FastQCFastQC Report
Fri 10 Feb 2017
SRR3557841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427740
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401121.6522362361702654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181340.7469498381210509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166870.6873470800003295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81320.3349617339583316No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54650.22510647762940017No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA34430.14181914043513721No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34340.1414484252844209No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.1386062757955959No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28250.11636336675261766No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA27350.11265621524545462No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26650.10977287518432781No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24720.10182309473007818No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA24490.10087571156713651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5900.059.5453531
TAACGCA6750.045.807734
GTATAAC8150.040.91461
AACGCAG356800.040.44686
TACAACG5200.040.030722
GTATCAA370450.037.885921
TAGTACT9550.037.3583035
AGATTAC12700.036.995772
ATAACGC8200.036.987013
CGCAGAG407700.035.5138248
ACGCAGA407550.035.4247677
CATGGGG93350.034.7790344
CATGGGT34400.034.7437634
GTACATG352000.034.729151
TACATGG352300.034.6074752
ACATGGG348150.034.4192623
ACAACGC5900.034.2704473
GCAGAGT424600.034.044289
TATCAAC410800.034.0224882
ATCAACG417550.033.4127663