FastQCFastQC Report
Fri 10 Feb 2017
SRR3557839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5797646
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1004581.7327377352808364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447480.7718304981021608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366560.6322566089754359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207920.3586283122494888No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA116750.20137483385498184No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102410.1766406572598603No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87320.15061285218173032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT77500.13367494324420637No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77120.1330195048128154No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA74650.12875915500877425No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC71470.12327417024081842No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA67300.11608159587529145No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA66530.11475347063273611No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC64310.1109243303230311No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60120.10369725919795725No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.10233463719585502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11400.056.395241
AACGCAG886550.042.0381246
TACAACG11000.041.627042
GTATCAA911100.040.1624951
ACGCAGA1006150.037.0233737
CGCAGAG1006750.036.989498
CATGGGT111500.035.9420364
TATCAAC1029000.035.3682482
GCAGAGT1052450.035.321179
ATCAACG1048300.034.640963
TCAACGC1074350.034.2967764
CAACGCA1087350.033.8593985
ACAACGC13750.032.8668823
ATGGGTG65250.032.4404645
TAGTACT20550.032.1165435
ACATGGG837100.032.0011063
GTACATG861800.031.9883481
TACATGG860500.031.9692382
ATAGTAC20750.031.8069864
CATGGGG210850.031.4708294