FastQCFastQC Report
Fri 10 Feb 2017
SRR3557838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895894
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124091.3850968976240492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65510.7312248993742564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.7192815221443608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29220.32615465668929583No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22980.2565035595728959No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20200.2254731028447562No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14350.16017519929813126No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA12820.14309728606286012No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11960.13349793613976652No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.12735881700290436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.12278238273724347No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.11887567055924027No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.10682067298140181No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.10369530323899925No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9110.10168613697602619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2750.067.121811
TACAACG2000.056.496892
AGATTAC4600.050.4205672
GTATAAC2650.047.1854481
TAGTACT3800.046.9424675
ATAGTAC4100.044.9579054
TATCACG2200.043.251212
GGTTTCA5250.043.098181
TAACGCA3050.040.9399874
CATGGGT13750.039.784454
GTACAAC3400.038.5281751
TCACGCA2500.038.054694
GCATAGT4900.037.624142
ATGGGTG7800.037.3533635
TTCAACG6450.036.87474
ACATGGG131800.036.140473
GTACATG137300.035.8649021
TACATGG138050.035.7124022
GATTACT6500.035.680263
CATGGGG30350.035.6566854