FastQCFastQC Report
Fri 10 Feb 2017
SRR3557837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040603
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258551.2670274423785517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127330.6239822248619649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123640.6058993346574517No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56090.2748697321330999No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37870.18558239892815995No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34150.1673524933561305No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28970.14196783989830458No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT28220.13829245571039542No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC25730.12609018020653698No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23670.11599512497041314No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC22870.11207471516997672No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA21660.1061450953468166No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT21630.10599807997930023No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA20760.10173463432132561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5000.048.8253751
TAACGCA5500.047.5738224
GTATAAC6200.045.1375431
TACAACG3950.042.1550522
GACCGTT4000.038.653737
AGATTAC10750.037.064232
CATGGGT36600.036.557754
CATGGGG71400.035.8136254
GATTACT11450.034.7982983
ACATGGG282350.033.6993033
GTACATG294050.033.5126341
TACATGG293700.033.4701422
AACGCAG278000.033.3273476
ATGGGTG20850.032.514485
TACTGGT13750.032.4374922
GTATCAA279050.032.326771
AAACGCA6150.031.9092715
GTACTGG15500.031.8843941
CATGGGC64300.031.3521084
ACAACGC4950.031.2361013
Produced by FastQC (version 0.11.5)