FastQCFastQC Report
Fri 10 Feb 2017
SRR3557831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208602
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479401.494108649187403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215220.6707594148479619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192700.6005730844772895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99570.31032206549768404No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA65430.2039205859748264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46510.14495409527264522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45060.14043499318394742No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA39900.12435322299244345No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC38760.1208002737640879No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA38700.12061327643627971No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38470.11989645334634835No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC38060.1186186382729924No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA36070.11241656023402093No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34790.1084272839074463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7800.053.4196471
AACGCAG444900.040.3596886
TACAACG6700.039.0500642
GTATCAA465950.037.8648721
TAGTACT11700.036.0807655
CGCAGAG506500.035.5450978
ACGCAGA505550.035.4825177
ATAGTAC11850.035.12234
CATGGGG106300.034.1192784
ACATGGG426400.034.0516783
GCAGAGT531450.033.8204239
TATCAAC522950.033.5774042
GTACATG445350.033.4546851
TACATGG447150.033.3650132
ATCAACG525550.033.340813
TCAACGC535850.033.0323074
ACAACGC8150.032.8295483
CAACGCA542350.032.691235
CATGGGT45200.031.7016534
CATGGGC113050.031.3457724