FastQCFastQC Report
Fri 10 Feb 2017
SRR3557822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4966538
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT768101.5465501321040935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338310.6811787204688659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303680.6114520819129945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144120.290182014111238No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90290.1817966559402143No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68240.13739953263218765No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58670.1181305770740101No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54220.10917061341320655No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA54090.10890886166581228No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52790.10629134419186967No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC51420.10353288346933015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8450.045.094111
AACGCAG641800.041.5973476
GTATCAA652850.040.4278681
ACGCAGA727700.036.6952327
CGCAGAG731450.036.5314948
TATCAAC743800.035.3648342
ATCAACG752200.034.8323363
GCAGAGT769050.034.768619
TCAACGC766500.034.597264
CAACGCA775350.034.1640175
GTACATG611700.031.3897421
ACATGGG594600.031.3633613
TACATGG615350.031.1686762
CATGGGT63600.030.9449564
TACAACG9900.030.0362452
CATGGGG157450.029.9089764
GACCGTT9950.029.2814127
ACAACGC11200.028.1403373
GGACCGT13750.028.107986
AGAGTAC761550.027.56882910-11