FastQCFastQC Report
Fri 10 Feb 2017
SRR3557819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12286852
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1572291.2796524284658104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710860.5785534000083992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT647800.5272302457944477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279730.2276661263601124No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC186420.1517231590321101No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC181790.14795490333895125No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT169430.13789536978226807No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA142830.11624621180429291No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138870.11302325445118082No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA137830.11217682120692916No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT131310.10687033586796682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1119000.052.3662261
AACGCAG1265950.045.888056
TATCAAC1367150.042.7757342
ATCAACG1384000.042.118663
TCAACGC1408100.041.4793784
CAACGCA1427950.040.773695
ACGCAGA1459350.039.7293367
CGCAGAG1461500.039.658688
GCAGAGT1536250.037.7444739
GGTATCA616150.036.1648981
GTACATG1175350.030.7045381
ACATGGG1155550.030.5616073
TACATGG1183900.030.414632
AGAGTAC1502900.030.25398410-11
GTACTGG99100.029.7361971
CATGGGT174600.028.571914
TACTGGT86250.028.5446472
CATGGGG388550.027.0249794
CATGGGA513800.026.9176544
GGACCGT42300.026.7075966
Produced by FastQC (version 0.11.5)