FastQCFastQC Report
Fri 10 Feb 2017
SRR3557818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4879326
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675021.383428776843359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305000.6250863336452617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278580.570939510907859No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137180.2811453877031377No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA79190.16229700577497794No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75680.15510338927958492No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65740.1347317231929164No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.11755721999308921No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC56130.11503638002461816No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55030.1127819702967172No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA49610.10167387872833257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10800.055.1179281
AACGCAG648200.038.262726
GTATCAA656250.036.755031
TAACGCA10450.035.2793124
TATCACG7850.034.0907442
ACGCAGA731100.033.8590137
CGCAGAG734250.033.7947358
ACGGTAC11450.032.7201543
TATCAAC744950.032.2194142
GCAGAGT771550.032.122429
ATCAACG746550.032.0617523
TCAACGC761800.031.90914
CAACGCA768150.031.7149935
TAGTACT19500.031.4085045
ATAGTAC19850.031.1542634
ACAACGC10950.030.4126433
ACATGGG602500.029.7683353
GTACATG636150.029.2794341
AGATTAC22800.029.2130432
CATGGGG156950.029.2104154