FastQCFastQC Report
Fri 10 Feb 2017
SRR3557811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3167357
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT616051.9449970432761448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283880.8962677715205455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238820.7540040481701306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126930.40074421670812604No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA82920.26179556014683536No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52530.16584805565018404No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA52530.16584805565018404No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50840.16051237672292704No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA45700.1442843354885477No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45680.14422119135923106No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43080.13601245454806643No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA41550.13118192865534262No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA38950.12297319184417796No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC35700.11271227083022217No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC34450.10876576274793147No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.10835532590737325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9400.048.1421431
AACGCAG521150.040.8840876
ACAACGC6950.039.3614163
ATAGTAC11950.037.819524
TAGTACT12550.037.432915
GGTTTCA15850.036.815991
GTATCAA565200.036.7779351
TAACGCA8050.036.1968234
CGCAGAG590100.036.1070028
ACGCAGA591000.036.0117727
AGATTAC16250.035.5006752
TACAACG7650.034.983992
GTATAAC9550.034.9159851
GCAGAGT618900.034.455629
ATAACGC9250.033.4317673
ACATGGG472050.033.297133
CATGGGG104550.033.160024
TACATGG487050.033.066892
GTACATG486750.033.041391
TATCAAC627400.032.9593352
Produced by FastQC (version 0.11.5)