FastQCFastQC Report
Fri 10 Feb 2017
SRR3557809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583223
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT824631.7992360397912126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332810.7261483894630482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322370.7033696592987075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148800.32466236096301665No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA103240.22525633162514677No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76420.16673855930640946No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70840.15456372077029637No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66780.14570532570638609No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62090.13547235209807595No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA59790.13045404947566375No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA59680.13021404369807013No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54820.11961015206984256No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA52780.11515913583083345No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC51660.11271544064078924No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC50670.1105553886424466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9800.047.9888571
AACGCAG744050.040.923746
GTATCAA785350.038.1810721
CGCAGAG841650.036.22058
ACGCAGA842350.036.1692287
AGATTAC19950.035.4726032
CATGGGT89700.035.2648354
GCAGAGT879150.034.668769
TATCAAC872150.034.31142
ATCAACG888500.033.6038173
TATCACG8500.033.582372
TCAACGC906000.033.3263054
CAACGCA916850.032.8411255
ATGGGTG52600.032.4428375
CATGGGG176150.031.9661254
TAACGCA10300.031.7503224
ACATGGG711650.031.6769353
TACATGG730200.031.4853882
GTACATG730050.031.4511451
TACAACG8450.030.965992