FastQCFastQC Report
Fri 10 Feb 2017
SRR3557808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973890
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355581.195673007407806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158980.5345860136050762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147120.49470558763101524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74350.2500092471476753No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63950.21503821593939254No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55250.18578360329400212No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42660.14344847993705215No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC37910.1274761339524999No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC36280.12199509733043254No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.11009149632299783No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31610.10629175927825171No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT31060.1044423297431983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5950.060.0308341
TACAACG5200.037.7370952
AACGCAG388450.034.9767726
ATAGTAC10550.033.8164064
GTATCAA396550.033.447011
TAGTACT10700.033.3423465
CATGGGG103250.032.0770034
ACGCAGA436100.031.1823437
CGCAGAG439050.031.0540838
TAACGCA5950.029.980094
ACATGGG379850.029.914693
TATCAAC442600.029.8531742
ATCAACG442450.029.7945923
TCAACGC451350.029.6940294
GCAGAGT459700.029.6073769
ACGGTAC7850.029.541393
GTACATG402600.029.3808381
CAACGCA456850.029.310515
TACATGG401800.029.2882692
CATGGGT35850.028.6936364