FastQCFastQC Report
Fri 10 Feb 2017
SRR3557806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022592
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT621401.5447751101777163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296260.7364903027699553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252400.6274561277902407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129940.32302555168408825No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80160.19927449763734428No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA75810.1884605746742399No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65710.16335238572542282No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56790.14117762875280415No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC53980.1341920831145689No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC48700.12106621800073186No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.1129371310836396No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA45340.11271339474647193No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT44380.11032687381668338No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA43270.10756745899161535No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA41090.10214806771355386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8600.059.5296251
ATAGTAC13050.038.7287834
TAGTACT13500.037.437825
TACAACG8350.037.0336272
TAACGCA9050.036.792974
AACGCAG609250.035.866356
GTATCAA628650.033.9289971
GCATAGT15550.033.6536142
CGCAGAG680100.032.2261168
ACGCAGA679550.032.18227
CATGGGT46100.032.1161774
GGACCGT13350.032.068356
GTATAAC11300.031.608651
ACAACGC9750.031.104513
GCAGAGT712850.030.7539149
ACATGGG546350.030.692763
CATGGGG132100.030.2926774
ATAACGC10850.030.1432973
TATCACG7700.030.1198832
TATCAAC705100.030.0584352
Produced by FastQC (version 0.11.5)