FastQCFastQC Report
Fri 10 Feb 2017
SRR3557797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403415
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144081.0266385922909473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74340.5297078911084747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63910.45538917568930076No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39180.27917615245668603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30430.21682823683657365No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27010.1924591086741983No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20730.1477111189491348No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT19620.13980184051046912No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19210.1368803953214124No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18390.13103750494329902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.12448206695809863No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA16550.11792662897289825No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG14850.10581331965241927No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA14220.10132426972777119No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3550.072.0724261
TAACGCA4800.049.5458534
TACAACG3100.046.0296942
TAGTACT6150.042.5369265
GTATAAC5900.040.3401071
TCACGCA2700.039.636684
AGATTAC8750.039.4101832
ATAGTAC6700.039.0450864
ACAACGC4000.037.1593863
AAACGCA4700.036.6850135
GATTACT10050.034.312353
GCATAGT7750.032.987952
CATGGGC44950.032.802774
TATCACG2750.032.430012
CATGGGT22750.031.6222294
ATAACGC8350.030.6175563
CATGGGG45450.030.479694
TACGCAG2550.030.3104025
ACGCATA1203.695886E-529.750831
ACATGGG206550.029.2453653
Produced by FastQC (version 0.11.5)