FastQCFastQC Report
Fri 10 Feb 2017
SRR3557788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923958
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231001.2006499102371258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114210.5936200270484076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97490.5067158430693394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50930.26471471830466153No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA36190.18810181927048303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33220.17266489185314857No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA26070.13550191844104706No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24620.12796537138544606No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.11902546729190555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.10914999183973871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5700.068.916731
TACAACG4350.062.872562
ACAACGC5800.047.1531943
AAACGCA6550.045.384755
TAGTACT9200.042.6518025
ATAGTAC9600.042.113274
AGATTAC10950.041.808942
GTATAAC6450.039.679331
GTACAAC7300.039.1357771
GCATAGT11200.036.0980262
TAACGCA6650.035.7618185
GATTACT13150.034.8133853
TACGCAG5350.034.4499975
GGACCGT5400.034.1310166
ACGGTAC3150.033.973733
GGTTTCA11350.033.5613671
AACGCAG267150.033.560426
ATAACGC8150.032.8273773
ACATGGG263000.032.7788353
CATGGGC67350.032.7504734