FastQCFastQC Report
Fri 10 Feb 2017
SRR3557780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7435272
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1108241.4905170920445143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550960.7410085333798144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427330.5747335134477931No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227740.3062967972119917No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA146900.19757179024519883No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112050.15070060651446243No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA95060.12785006385778488No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91520.1230889737456814No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA90880.12222821169151579No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT88640.11921554450193617No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA83340.11208735874087727No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77350.10403116389017107No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA77070.10365458049147361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG16400.053.714821
AACGCAG997800.040.1027766
TACAACG14350.036.8779952
GTATCAA1067150.036.622811
TATCACG12850.035.630082
ACGCAGA1129450.035.386247
CGCAGAG1134400.035.2899558
GCAGAGT1196100.033.4695479
ATAGTAC27700.033.0555084
TAGTACT28150.032.527095
AGATTAC29700.032.4330442
ACAACGC16750.032.3020023
TATCAAC1212400.032.040252
TAACGCA15850.031.885454
ATCAACG1217900.031.7813433
TCAACGC1244950.031.5683924
CAACGCA1260750.031.2859525
CATGGGT104500.030.6672884
CATGGGG234200.030.3123974
ACATGGG915000.030.2158433
Produced by FastQC (version 0.11.5)