FastQCFastQC Report
Fri 10 Feb 2017
SRR3557769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194919
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT716171.1560603132986889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347040.5602010292628523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272040.4391340710023811No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143000.23083433374996512No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA90820.14660401532287992No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74140.11967872380575113No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64150.10355260496545636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG14150.044.5864561
AACGCAG670950.038.2383166
GTATCAA708450.035.0248451
ACGCAGA752850.033.9679347
CGCAGAG762250.033.6582458
TACAACG11250.033.2965852
TAACGCA14000.032.277044
GCAGAGT802250.031.9800539
ACATGGG615800.031.0999133
CATGGGG177650.030.9588384
TCAACGC816200.030.7054
ATCAACG800700.030.6607783
GTACATG651750.030.5926631
TATCAAC806450.030.545632
TATCACG9350.030.5239852
CAACGCA830200.030.351935
TACATGG654300.030.3424852
AGATTAC28850.029.0592862
ACAACGC14800.028.1218873
TAGTACT24500.027.4233515
Produced by FastQC (version 0.11.5)