FastQCFastQC Report
Fri 10 Feb 2017
SRR3557765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5993750
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624011.0411011470281544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283310.47267570385818564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232860.3885046923879041No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA192440.3210677789363921No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT176570.2945901981230448No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT127690.21303858185610008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124970.2085005213764338No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG85270.14226485922836288No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74460.12422940563086549No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA73320.12232742440041709No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC65590.10943065693430658No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60430.10082168925964546No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10900.047.508781
TAACGCA13250.035.0039984
AGATTAC28750.031.4375212
TATCACG9650.031.4257322
CATGGGT90900.031.2682044
AACGCAG843250.029.0028886
GATTACT31150.028.6333483
GTATCAA838200.028.0214461
CATGGGG173450.027.9397324
CATGGGC160300.027.6722344
TACAACG11100.027.320572
ACGCAGA931150.026.2011767
CGCAGAG934550.026.1949318
ACATGGG826750.025.9640183
ACAACGC12900.025.8129253
GTACATG870850.025.556021
TACATGG873700.025.4673632
GCAGAGT978450.025.0196469
GTACTGG37850.025.0041681
TCAACGC956600.024.9384524
Produced by FastQC (version 0.11.5)