FastQCFastQC Report
Fri 10 Feb 2017
SRR3557761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4137657
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT666231.610162466342667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317990.7685267290159624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244150.5900682439361213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144150.34838557183449476No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA91860.2220097025925542No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79630.19245191179452525No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69900.168936187799037No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68350.16519010638146178No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA57830.13976508927637066No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56730.13710657988325278No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA51720.12499827801096128No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA50400.1218080667392198No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA47120.11388087509428646No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG46370.11206825505352426No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44600.10779047175732547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.104600260485584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10900.067.1615451
ATAGTAC16350.045.0914734
TACAACG8350.044.1501772
TAGTACT17450.041.9083145
GCATAGT18350.040.5043032
ACAACGC10900.037.6368373
AGATTAC22300.037.5959852
AACGCAG632450.037.4433446
TATCACG8000.037.1626972
TAACGCA10400.037.1595574
GATTACT24050.034.610153
CGCAGAG711100.033.3776368
GTATCAA689850.033.285081
ACGCAGA711750.033.2799267
GTATAAC12750.033.142881
CATGGGT58900.032.907344
CATGGGG129550.032.676314
AGTGTAC23550.032.5678633
TCACGCA9400.032.2576564
AAACGCA13800.031.8818235