FastQCFastQC Report
Fri 10 Feb 2017
SRR3557760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9307165
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1195561.284558724380625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598140.6426661609630859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT433760.4660495435505871No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244570.26277604404778465No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA146060.15693285764247222No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT144320.15506333024073388No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA142630.15324752489076965No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124450.13371418686571046No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104050.11179558974188167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG16650.040.037741
AACGCAG1153800.038.7375036
TAACGCA18650.036.982544
GTATCAA1207500.036.1509361
ACGCAGA1296350.034.4273727
CGCAGAG1303500.034.3301288
TACAACG16600.032.9540632
GCAGAGT1373000.032.5663839
CATGGGG284050.031.9012554
TATCAAC1378950.031.5682962
ATCAACG1383750.031.3591163
TCAACGC1410100.031.2453844
CAACGCA1425600.031.0015935
ACATGGG1098300.029.6564123
GTACATG1138000.029.46731
TACATGG1144500.029.1977752
TATCACG16150.029.0859852
CATGGGT120250.028.9264
GTATAAC25800.027.6839031
ACAACGC21350.026.7356623