FastQCFastQC Report
Fri 10 Feb 2017
SRR3557756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702843
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269831.5845853082169057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136030.7988405272829027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101940.5986459115725877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53840.31617712261200825No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA41010.24083253711587033No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37500.2202199498133416No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28310.16625138077908533No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA26710.15685532958704942No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.1476941796748144No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22380.13142726604860225No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.11856642097950311No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.11739191458049862No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA19240.11298751558423178No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA19030.11175428386527707No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18040.10594047719020484No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.10453106951139945No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.10235823267324116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5350.068.976871
TACAACG3950.051.1740382
TATCACG3300.045.0395432
AGATTAC9750.043.903162
GTATAAC5750.043.4757421
ATAGTAC8650.042.6131364
TAGTACT8700.042.3682335
TAACGCA6000.041.616544
ACAACGC4950.040.835853
GATTACT11100.039.0991943
AGTGTAC8550.038.939453
ACGCATA2000.038.6882321
GTACAAC6050.038.3684921
TTCAACG12150.036.6988874
GCATAGT9900.036.632162
AACGCAG276500.036.2303626
GGTTTCA11550.036.0729451
ATAACGC7600.033.6374243
CATGGGC64200.033.337684
ATCACGC4500.033.0293
Produced by FastQC (version 0.11.5)