FastQCFastQC Report
Fri 10 Feb 2017
SRR3557755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247899
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327421.0080978503334002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147630.4545399964715651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119620.3682996300069676No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73470.22620777308653994No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63050.19412549466593634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57550.1771914705475755No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA39200.12069340826177169No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36870.11351953986253882No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32870.10120388595827642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6850.040.8315931
TACAACG7000.036.5217322
TAACGCA7950.035.896194
ACAACGC7550.033.073233
AACGCAG410200.032.944136
AAACGCA11000.031.3479825
CATGGGT35600.030.7285524
GTATCAA422050.030.4423221
TATCACG6000.029.7269942
ACGCAGA455850.029.5667697
CGCAGAG459050.029.451328
CATGGGG101500.028.9357814
ACATGGG374200.028.1059533
GCAGAGT482650.028.04829
GTATAAC11300.027.3850731
TCAACGC483850.027.0079254
ATCAACG475150.026.9644033
GTACAAC11700.026.9574621
TATCAAC477150.026.9265542
TACATGG413150.026.895682