FastQCFastQC Report
Fri 10 Feb 2017
SRR3557752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5081869
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551631.0854864617722337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266580.524570782914711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201480.39646830723106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99030.19486924987637422No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60490.11903101004768128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA464500.042.5172771
AACGCAG481000.042.2944876
ACGCAGA548950.037.0267147
CGCAGAG552750.036.9227378
ATCAACG541650.036.2745133
TCAACGC554500.035.948514
TATCAAC547750.035.9262242
CAACGCA562950.035.4828345
CGCATAG9300.035.200961
GCAGAGT590200.034.519449
ACATGGG458400.032.785463
GTACATG474800.032.5814441
TACATGG480450.032.1977162
CATGGGG148550.029.9348984
CATGGGT67000.028.8374864
AGAGTAC576900.028.40116110-11
GGACCGT11700.027.9463866
TAACGCA10200.027.3934384
CATGGGC121450.025.9923694
AGTACTT357200.025.93179912-13
Produced by FastQC (version 0.11.5)