FastQCFastQC Report
Fri 10 Feb 2017
SRR3557751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237420
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240691.0757479597035873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118090.5277954072100902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92630.4140036291800377No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44250.19777243432167407No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40640.18163777922786065No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31300.13989326992696946No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29060.12988173878842596No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC26720.1194232642954832No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24940.11146767258717631No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT22860.10217125081567163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5250.053.2904431
TAACGCA4850.045.354174
ACAACGC5050.037.6717533
TATCACG4500.035.670442
CATGGGT37050.034.659544
AACGCAG270400.034.2545286
TCACGCA4800.033.441044
AGATTAC10550.033.247332
GTATCAA269000.032.994091
ATAACGC6850.032.979973
GTATAAC7050.032.929591
ACATGGG255300.032.647843
ACGCATA2750.032.4690321
TACAACG5950.031.9735072
GTACATG277700.031.2744881
TAGTACT8950.031.219945
ATCACGC5150.031.1683463
TACATGG280200.031.0832712
ACGCAGA301350.030.8350737
CATGGGG67300.030.7412414