FastQCFastQC Report
Fri 10 Feb 2017
SRR3557750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4948253
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442370.8939922837413526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217100.4387407030319589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160420.32419522607271695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73930.14940626520107197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA375950.043.399671
AACGCAG414300.040.310426
CGCATAG6900.038.8213731
ACGCAGA466250.035.7934957
ATCAACG453950.035.6893843
TCAACGC461900.035.680084
TATCAAC456500.035.5182232
CGCAGAG473300.035.2728968
CAACGCA470700.035.1014375
GCAGAGT505150.033.0489239
GTACATG414750.031.2449251
TACATGG418450.030.861932
ACATGGG406450.030.8496323
TAACGCA7900.030.1032544
TATCACG6850.028.6437742
AGAGTAC489400.028.19176710-11
CATGGGT74150.027.5019534
CATGGGG144800.026.9759864
AGTACTT283300.026.2358112-13
ACAACGC9750.026.2207053
Produced by FastQC (version 0.11.5)