FastQCFastQC Report
Fri 10 Feb 2017
SRR3557749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697525
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253080.9381933438985737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122020.4523405714497549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93230.3456131083122492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.1731587288347652No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42840.15881224455751103No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31330.11614350191379134No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA28910.10717231536315697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6650.051.9112851
ACAACGC5350.046.667153
TAACGCA5300.040.3777774
TACAACG6250.039.94932
TATCACG4300.038.7105642
ATAGTAC10150.035.139944
AACGCAG296150.035.0668646
GTATCAA287050.034.792841
TAGTACT10650.032.9320035
ACGCAGA330100.031.568377
CGCAGAG332500.031.4656548
GCATAGT12500.030.9131492
TCAACGC334150.030.2073064
ACATGGG274350.030.2046363
ATCAACG327050.030.172393
TATCAAC329600.029.9766942
CAACGCA339300.029.9415285
GCAGAGT350550.029.7437279
ATCACGC6000.029.7225283
GTACATG297550.029.0043741
Produced by FastQC (version 0.11.5)