FastQCFastQC Report
Fri 10 Feb 2017
SRR3557748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017701
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76240.7491394820286115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38650.37977755745548053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.3191507132252007No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20490.20133614882956782No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.15279536917031622No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13720.13481366334512787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2000.047.5639232
CGCATAG2700.041.8859631
TACAACG1800.039.63662
TAACGCA2350.037.9499364
GTACTAA1104.6856803E-737.8777351
ATCACGC2950.034.2621463
TCACGCA2950.032.2467234
CATGGGG31600.031.6089324
CATGGGT11800.030.7351594
GTATCAC3900.030.5241831
ACATGGG109350.030.502243
GTACATG116100.030.2481251
AACGCAG107750.030.0723176
TACATGG116650.029.8166472
TAGTACT4050.029.3604455
GTATCAA106450.029.2997761
ACAACGC2502.3646862E-1128.5383513
GTGTTAT4650.028.161021
ATAGTAC4200.026.8962654
CGCAGAG120950.026.8886578
Produced by FastQC (version 0.11.5)