FastQCFastQC Report
Fri 10 Feb 2017
SRR3557747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325391
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330280.9932065131589037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159660.4801239914343908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118730.3570407209257498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58970.17733253021975462No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55470.16680745211615716No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42730.12849616781906248No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC37410.11249804910159436No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC34380.10338633862905144No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT33380.10037917345659504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6850.045.177841
GTATCAA344350.037.3480071
AACGCAG364600.035.989336
ATCAACG393400.032.3873833
ACGCAGA403800.032.3777777
CGCAGAG405800.032.203558
TATCAAC397800.032.1347432
TCAACGC400700.032.123784
CATGGGT51750.031.9390894
CAACGCA406850.031.7404865
CATGGGG97750.031.6890374
ACATGGG344750.031.4908663
GCAGAGT427650.030.5442649
GTACATG376400.030.2150861
TACATGG378350.030.1408842
ACGGTAC6900.026.7116493
AGTACTT216550.025.6995412-13
CATGGGC77750.025.617254
GAGTACT190150.024.811812-13
AGAGTAC419000.024.669410-11
Produced by FastQC (version 0.11.5)