FastQCFastQC Report
Fri 10 Feb 2017
SRR3557746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390150
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559711.0383941077706558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265620.49278777028468596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201020.37293952858454776No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT108380.20107047113716686No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC106540.19765683700824652No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC91930.17055183993024312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91180.16916041297552015No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT76270.14149884511562758No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT69460.12886468836674303No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA68740.127528918490209No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC56550.1049135923861117No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA54510.10112891106926523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA451050.048.7702981
AACGCAG527050.042.4442256
TATCAAC550250.039.7814032
ATCAACG557600.039.1272663
TCAACGC568650.038.795574
CAACGCA578200.038.226765
ACGCAGA597250.037.3359457
CGCAGAG600600.037.1771858
CGCATAG8250.036.0715031
GACCGTT13950.035.3704077
GCAGAGT633650.035.2662359
GTACTGG49200.033.9929921
TACTGGT46100.033.400672
TAACGCA7450.032.7162744
ACATGGG510300.031.7684333
GTACATG524000.031.7580661
TACATGG526150.031.6037962
CATGGGG192300.030.666814
AGAGTAC625200.028.65989510-11
GGTTCAC51450.028.5395956