FastQCFastQC Report
Fri 10 Feb 2017
SRR3557745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886124
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324611.1247264497298106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159110.5512930144373561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116530.4037595058285784No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA71640.2482221831078637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63750.22088448036189715No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT56590.1960761214694864No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38620.13381268441688576No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33110.11472133560443001No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32340.1120533975671177No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31000.10741049241127547No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30380.10526228256304995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7100.053.6478841
TATCACG6700.041.7056472
TAACGCA7350.038.82564
TCACGCA7400.037.759864
ATAACGC8300.035.8142783
CATGGGT38800.033.55654
ATCACGC8400.033.264643
CATGGGG80200.032.913414
AACGCAG383600.032.5000536
GTATAAC9050.032.223931
ACAACGC7100.030.981873
TACAACG7500.030.9154192
GTATCAA394000.030.3469261
ATCTACG5550.029.9941712
AGATTAC14450.029.6235052
ACGCAGA424000.029.3192237
ACGCATA3250.029.3000031
CGCAGAG425000.029.222268
ACATGGG350250.029.0086963
GTATCAC11300.028.9677831