FastQCFastQC Report
Fri 10 Feb 2017
SRR3557744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269754
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422331.2916262201988282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205330.6279677309057501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152020.46492794259139986No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93110.2847614835856153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.23179113780425073No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT73930.2261026364674529No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC54410.16640395577159628No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA46540.14233486678202703No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC42340.1294898637634513No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG41800.12783836337534873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37840.11572736052926305No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT37300.11407586014116047No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36860.11273019315826205No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36770.1124549430935783No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA34510.10554310813596375No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33440.10227069070027899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6550.038.1598661
TAACGCA6850.036.4545944
AACGCAG497600.033.1809656
GTATCAA489050.032.8555951
GTATAAC9150.031.8693961
TATCACG6050.030.4653722
TACAACG6050.030.4653722
CATGGGG103250.030.404474
CGCAGAG545750.030.26448
ACGCAGA545100.030.2132287
ATAACGC8750.029.2182353
TATCAAC554450.028.8784682
GCAGAGT571200.028.8535089
ATCAACG558350.028.5591853
TCAACGC569150.028.497794
CAACGCA572900.028.4046555
GACCGTT6350.028.0893087
GTACTGG22950.028.005281
GGACCGT10250.027.8426726
ACGCATA3000.027.7719021