FastQCFastQC Report
Fri 10 Feb 2017
SRR3557743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689761
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424521.5782814904372544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197530.7343775153257112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156670.5824681077612472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72610.26994963493038976No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC50140.18641061417724475No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48700.18105697866836495No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC46870.17425340020916358No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45690.16986639333383152No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41130.15291321422237886No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36610.13610874720839508No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.13224223267420412No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA33170.12331950682607117No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT28870.10733295634816624No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA27800.10335490774087362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA363550.041.8533171
AACGCAG383750.040.2168126
TATCAAC420300.036.125422
ATCAACG425400.035.7062953
ACGCAGA433200.035.6397677
CGCAGAG434400.035.5960588
TCAACGC433150.035.259584
CAACGCA437750.034.9162255
CGCATAG4800.034.7120361
GCAGAGT455300.033.975129
GACCGTT6000.033.6882937
ACATGGG336000.032.5559163
CATGGGT30000.031.9047974
GTACATG360600.031.436381
TACATGG359950.031.3972282
CATGGGG97000.030.7055824
TCACGCA4650.030.6838364
TATCACG4500.030.3855192
GGACCGT10400.030.29666
GTACTGG21100.028.7661021