FastQCFastQC Report
Fri 10 Feb 2017
SRR3557741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328185
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327280.9833587976629905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153290.46058136792275667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119130.3579428427205819No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56920.17102414679472444No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41740.12541370146190792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA301300.040.812871
AACGCAG315550.039.7178086
ACGCAGA353050.035.3980527
CGCAGAG358600.034.93318
TATCAAC352550.034.6387832
TCAACGC357150.034.5255784
ATCAACG353700.034.492543
CAACGCA361750.034.1687285
GCAGAGT380050.032.8989039
ACATGGG308150.031.1596973
GTACATG320750.030.9895861
TACATGG324600.030.5147192
CATGGGG99700.029.9358394
CGCATAG6550.027.2612571
AGAGTAC369250.026.99513410-11
AGTACTT202600.026.56078712-13
TAACGCA5900.026.2001764
CATGGGT38600.026.0304874
CATGGGC76050.025.720514
GAGTACT182050.024.95367412-13
Produced by FastQC (version 0.11.5)