FastQCFastQC Report
Fri 10 Feb 2017
SRR3557740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4731188
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373190.788787086879659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184520.3900077528096538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138130.2919562697571942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63940.13514576043057264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53930.11398828370379702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA346050.040.0920141
AACGCAG384000.036.598696
ATCAACG410550.033.4354483
TATCAAC413750.033.2925072
TCAACGC417150.033.2912334
CAACGCA421450.032.979785
ACGCAGA424850.032.911757
CGCAGAG432150.032.369558
GCAGAGT462100.030.2844569
ACATGGG369900.029.620363
CGCATAG7500.029.3626271
GTACATG392400.029.1223681
TACATGG397350.028.726752
CATGGGG127900.026.5873344
AGAGTAC445250.025.43644510-11
CATGGGT50100.024.3257054
AGTACTT244850.024.29422212-13
GAGTACT214100.024.15953612-13
ACGGTAC7950.023.9294173
GGTATCA226900.023.8180271
Produced by FastQC (version 0.11.5)