FastQCFastQC Report
Fri 10 Feb 2017
SRR3557739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3440056
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426941.2410844474624831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199470.5798452118221331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157510.4578704532716909No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80210.23316480894497066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75000.21801970665593817No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65470.1903166692635236No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC53790.15636373361363887No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49260.14319534333162018No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC48920.14220698732811327No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT45090.13107344764155002No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38370.11153888192517797No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36470.10601571602322753No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA34430.10008558000218601No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9400.050.0103951
TACAACG6950.039.346942
ATAGTAC12450.038.199024
TAACGCA7950.035.8926664
ACAACGC7800.035.0586173
TATCACG7100.034.3291782
TAGTACT14250.034.208235
AACGCAG476350.033.533056
GACCGTT7750.032.9836247
GTATCAA471650.032.752611
TCACGCA7700.032.425764
CATGGGG111200.031.7550814
GTATAAC10600.030.3143961
ACATGGG436650.030.251173
ACGCAGA528800.030.1620467
CGCAGAG530800.030.0931978
CATGGGC94650.029.5194874
GCATAGT17050.029.2882082
CATGGGT39600.029.1231354
TACATGG472850.029.0168552
Produced by FastQC (version 0.11.5)