FastQCFastQC Report
Fri 10 Feb 2017
SRR3557737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646146
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234740.887101467568305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121580.4594606646798778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88960.3361870433453029No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47930.18113135102900596No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.16340746126630956No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36940.13959925113731442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6450.058.1299321
TATCACG3800.039.1154632
TAGTACT9700.037.388135
ATAGTAC10750.033.7362634
AACGCAG288700.033.484936
GTATCAA282050.032.600281
AAACGCA8400.032.557735
CATGGGT42050.031.2465344
ACATGGG273050.030.6139053
ACGCAGA318350.030.2542087
CATGGGG75100.030.0037044
CGCAGAG321900.029.9574938
ATCACGC4800.029.7266253
GTACATG297500.029.3068851
TACATGG297450.029.263022
GCATAGT12550.028.898692
TCAACGC323050.028.765034
ATCAACG319000.028.7388443
CAACGCA324800.028.701575
GCAGAGT338900.028.4372149
Produced by FastQC (version 0.11.5)