FastQCFastQC Report
Fri 10 Feb 2017
SRR3557736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775525
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260461.466946396136354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124560.7015389814280283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93900.5288576618183354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46580.2623449402289464No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45920.2586277298263894No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36710.20675574829979865No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32190.18129848917925684No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC29200.16445839962827896No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24410.13748046352487295No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23450.1320736120302446No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.13089086326579463No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT22820.12852536573689471No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA22010.12396333478830206No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20200.11376916686613818No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18950.10672899564917418No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT18900.10644738880049563No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17770.10008307402036018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3450.056.9410441
TAACGCA4250.046.161554
GACCGTT4350.039.633657
GGACCGT6050.039.3061036
TCACGCA3600.037.9822464
AGATTAC9000.037.651972
CATGGGT30700.036.9871064
TACAACG4100.036.250292
ACAACGC4350.035.533623
AACGCAG298550.034.668666
TATCACG3900.033.5361672
GTATCAA300800.033.089411
ACGCAGA329450.031.435047
CGCAGAG328900.031.4333788
GTATAAC6250.031.4314561
ATAGTAC6450.030.4165274
GCAGAGT340450.030.3844439
ATAACGC7650.030.3080883
GATTACT11050.030.128753
CATGGGG58800.030.0285574