FastQCFastQC Report
Fri 10 Feb 2017
SRR3557735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5872057
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT651671.1097814615900357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA362680.6176370563160405No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT336630.5732744079289421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319940.5448516593078031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234780.3998258191294805No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT174910.2978683619726444No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA155770.2652733105281505No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG139680.2378723503535473No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC127430.21701083623677356No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC119110.20284203644480972No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT118420.2016669797312935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112850.19218137698595228No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC110410.1880261039700398No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG109900.18715758379048433No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG91800.15633363231998598No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC89160.15183776315522823No Hit
CATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA88750.1511395410500954No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC77300.1316404115287028No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT72270.12307441838524387No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT70450.11997499343075178No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT67010.11411673967061287No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG66840.11382723294409437No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66260.11283950411244306No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA65490.11152820893938871No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC62420.10630005805461357No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA61160.10415430231688827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG14850.035.6694831
CATGGGG178450.029.1498494
CATGGGT77500.028.6122464
TACTGGT40150.027.3936252
GTACTGG47950.027.1824591
ATAGTAC20100.026.618974
ACGGTAC13900.026.5168363
TAACGCA10800.026.4217934
GGACCGT19700.026.2541436
TACAACG9650.025.8752942
AGTACTT436350.025.07102212-13
GGTTCAC43450.024.3542676
GAGTACT379050.024.16319712-13
CTAAGCA76800.024.1511734
GACCGTT12600.024.0627047
CATGGGC152450.024.0603854
ATGGGTG47400.023.9552025
TCTAAGC79900.023.8093763
TACTTTT489700.022.4797814-15
TAAGCAG87750.022.2891545
Produced by FastQC (version 0.11.5)