FastQCFastQC Report
Fri 10 Feb 2017
SRR3557734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933149
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266540.9087161954609193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123160.41989002263437697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98810.3368734421606267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45460.15498701225201994No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37430.12761029187402345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA260400.038.8515781
AACGCAG280800.036.9038286
TCAACGC308650.033.015334
ATCAACG304200.032.9901543
TATCAAC306650.032.8235172
ACGCAGA315100.032.811217
CAACGCA313400.032.685675
CGCAGAG319350.032.3187038
GCAGAGT338400.030.534489
CGCATAG3900.030.5187051
CATGGGT42600.030.284564
ACATGGG272400.029.8790973
GTACATG291250.028.9946041
TACATGG292800.028.8329032
CATGGGG89250.028.4440064
CATGGGC61900.025.0680734
AGAGTAC329550.024.97863410-11
AGTACTT170700.024.69651412-13
GAGTACT152950.024.08323312-13
GGACCGT7000.023.7810546
Produced by FastQC (version 0.11.5)