FastQCFastQC Report
Fri 10 Feb 2017
SRR3557733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110337
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235561.116219826501644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109060.5167894985492838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89040.42192313360377987No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.19276542087827678No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38570.18276701777962479No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29810.14125705989138226No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24580.11647428822979457No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC21980.10415398109401483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4450.045.4706421
TAACGCA4500.036.9899834
AACGCAG259450.035.7216076
TATCACG3400.034.970352
GTATCAA257000.034.8278661
CATGGGG66800.033.194874
CGCAGAG289350.032.0714078
ACGCAGA288200.032.034367
ATAACGC6600.030.6248233
TATCAAC291250.030.5565782
GCAGAGT303450.030.5420069
TCAACGC296750.030.4703254
ACATGGG235100.030.3941783
ATCAACG291750.030.3608553
CAACGCA300300.030.2288975
TACAACG3950.030.101062
ATTACGC1602.6246562E-729.7240923
GTACATG256850.029.1251321
TACATGG257950.029.039152
GGACCGT6600.028.8233626
Produced by FastQC (version 0.11.5)