FastQCFastQC Report
Fri 10 Feb 2017
SRR3557732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700115
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448881.2131514831295784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216250.5844412943922013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168810.45622906315073997No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA102500.27701841699514745No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT84290.22780373042459492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76970.2080205615230878No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC69320.1873455284497914No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54620.1476170335246337No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT51310.1386713656197172No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA47920.12950948821860941No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44700.1208070559969082No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG43710.11813146348154044No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40040.10821285284376297No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39870.10775340766435637No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38680.10453729140851027No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA37370.10099686090837717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8250.050.4988981
TAACGCA7000.034.8196564
ATAGTAC12250.034.4556924
AACGCAG532350.033.2110336
GTATCAA529300.032.8785971
TAGTACT13400.032.3859565
ACGCAGA588250.029.9641157
CGCAGAG590200.029.925558
TACAACG6500.029.2683872
TATCAAC598350.028.9134482
GCATAGT14850.028.8249282
TATCACG6200.028.766812
TTCAACG17600.028.7107774
ATCAACG600350.028.6868363
GCAGAGT618200.028.5797569
CATGGGG115450.028.5268964
TCAACGC612350.028.4838734
CAACGCA614050.028.3759755
CATGGGT41850.027.4157754
GTACTGG28400.026.8243661