FastQCFastQC Report
Fri 10 Feb 2017
SRR3557729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3707595
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260510.7026387725735955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129580.34949879908673953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99970.2696357072441839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44940.1212106500305454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38980.10513553934558657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA269650.039.001571
AACGCAG291400.036.9497876
TCAACGC315200.033.537344
ATCAACG312650.033.4305463
CAACGCA318500.033.2645235
CGCATAG5400.033.0653761
ACGCAGA327050.033.012987
TATCAAC318900.032.8694572
CGCAGAG334250.032.5686658
TAACGCA6500.032.013844
GCAGAGT355800.030.5960569
ACATGGG295050.030.3870643
GTACATG313750.029.839461
TATCACG5000.029.7279382
TACATGG314350.029.6759242
CATGGGG106350.029.1820684
CATGGGT45650.026.568834
AGAGTAC345300.025.11470210-11
GAGTACT159900.024.48874312-13
AGTACTT185350.023.86929112-13
Produced by FastQC (version 0.11.5)