FastQCFastQC Report
Fri 10 Feb 2017
SRR3557728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4802591
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT599101.2474516360023162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305080.6352404358397373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224860.46820559985224647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107810.2244829926179431No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA85470.17796643520133196No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74520.15516624255532066No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA61170.12736874741155346No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53130.11062778404407121No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC48520.10102879882963176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG594650.037.4005176
GTATCAA590450.036.928571
CGCATAG8300.036.5664021
ACGCAGA662200.033.5225037
CGCAGAG666350.033.3761838
GCAGAGT702000.031.6727549
TATCAAC686750.031.6319332
ATCAACG686400.031.5784443
TCAACGC698700.031.4309464
CAACGCA703500.031.2587455
CATGGGG143300.030.5339474
TAACGCA8250.029.5448114
ACATGGG536150.028.5967273
GTACATG563900.028.5150011
TACATGG565500.028.3323142
AGAGTAC687600.026.6733410-11
AGTACTT383650.026.46847312-13
GTACTGG29450.025.0568541
CATGGGC128650.024.6764564
CATGGGT53750.024.1117844