FastQCFastQC Report
Fri 10 Feb 2017
SRR3557726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5840884
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT775551.3277955871063352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369840.633191825073054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269790.46189926045441065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134630.23049593178018943No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA82050.14047531161378998No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68930.11801295831247462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA591050.046.464231
AACGCAG626750.044.2996866
TATCAAC703400.038.9573942
ACGCAGA713750.038.8999337
CGCAGAG716200.038.7170568
ATCAACG712100.038.4056323
TCAACGC723400.038.0851444
CAACGCA733650.037.5935675
GCAGAGT759600.036.481469
CGCATAG9500.032.5795561
GTACATG589500.032.2389221
ACATGGG580100.032.048163
TACATGG591700.032.0232962
AGAGTAC745550.029.697410-11
GGACCGT16250.028.9035916
CATGGGG176750.028.5242674
CATGGGA294550.027.4509894
AGTACTT452900.027.29964312-13
GACCGTT12150.026.9131477
ATGGGAC57850.026.7207155
Produced by FastQC (version 0.11.5)